| The Beneficial Effect of Korean Red Ginseng on Rat Bladder Contractility and Oxidant Damage Following Ischemia/Reperfusion. |
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| Ju Hyun Shin, Tae Hoon Oh, Seung Woo Yang, Chang Shik Youn, Young Ho Kim, Eu Gene Hwang, Yong Woong Kim, Seung Mo Yuk, Jae Sung Lim, Ki Hak Song, Chong Koo Sul, Yong Gil Na |
| Department of Urology, School of Medicine Chungnam National University Hospital, Daejeon, Korea. synthia2000@naver.com |
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| ABSTRACT |
PURPOSE
N-acetylcysteine (NAC) is a potent antioxidant, and a free radical scavenger. We investigated the possible effects of NAC after ischemia/reperfusion (I/R) of rat bladder.
MATERIALS AND METHODS
I/R injury was induced by abdominal aorta clamping and ischemia for 60minutes, followed by 120minutes reperfusion. Twenty rats were divided into four groups: sham operation + saline group (S+S), sham operation + NAC group (S+NAC), I/R + saline group (I/R+S), I/R + NAC group (I/R+NAC). Blood levels of reactive oxygen species (ROS) were determined using the free oxygen radical tests (FORT). Superoxide generation was measured based on lucigenin-enhanced chemiluminescence. The level of malondialdehyde (MDA) was analyzed in order to measure lipid peroxidation.
RESULTS
In I/R+S group, the isometric contractile responses to carbachol were significant lower than other groups and were reversed by the pretreatment with NAC. The level of FORT and MDA showed a marked increase in I/R+S group compared with S+S group. NADPH-stimulated superoxide production was also significantly increased. I/R+NAC decreased these parameters compared with I/R+S group.
CONCLUSION
Our results suggest that treatment with NAC reversed the low contractile responses of rat bladder and prevented oxidative stress following I/R. |
| Keywords:
Bladder; Ischemia; Reperfusion; N-acetylcysteine |
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